Make Every Cell Count
Discover in situ NGS library preparation for comprehensive and scalable multi-omic profiling at single-cell resolution
Achieve True Single-Cell
Multi-Omics with Our
Scalable In Situ Workflow
Biology is inherently diverse, yet most experiments rely on bulk measurements that average out the unique characteristics of individual cells. Single-cell sequencing technology addresses this challenge, but current platforms often lack the sensitivity needed to capture multi-omic details at scale. Consequently, researchers face trade-offs between sensitivity, depth, scale, and cost, making it challenging to conduct true single-cell studies across complex tissues or engineered cell populations.
At Factorial, we developed an in situ single-cell chemistry that enables NGS library preparation for all cells in a single tube, in parallel, prior to cell barcoding. This approach allows scientists to perform single-cell multi-omic experiments at a meaningful scale.
Resolve Every Edit, Every Construct, Every Outcome with Precision
Whether your team is iterating on a CRISPR therapeutic, optimizing a next-generation CAR design, or developing a novel AAV delivery modality, Factorial provides the sensitivity, resolution, and throughput needed to advance cell engineering programs. Our in situ single-cell workflow empowers cell engineering with single-cell clarity on:
Characterize Somatic Variation Across the Entire Body at Single-Cell Resolution
Somatic mosaicism provides a powerful lens for mapping somatic evolution across the entire body, revealing how mutations accumulate, how cells respond to environmental or carcinogenic stressors, how clonal dynamics shift with aging or early disease, and how co-occurring mutations shape lineage relationships and tissue heterogeneity. But until now, limitations in sensitivity, throughput, and cost have made large-scale single-cell studies difficult to execute.
With Factorial, your team can finally perform somatic mosaicism studies at meaningful scale.
Our platform enables:
- Single-cell DNA sequencing across hundreds of thousands to over one million cells
- Cost-efficient, profiling of somatic variation
- Tissue-wide and organism-wide mosaicism mapping
- Reconstruction of mutational phylogenies and evolutionary trajectories
- Detection of rare clones and low-frequency variants
- Applications spanning oncology, aging, inflammation, development, and environmental exposure biology
Explore A New Workflow for Scaled Discovery
Factorial’s proprietary in situ chemistry performs library preparation inside intact cells offering both the power of bulk NGS and the resolution of single-cell analysis, at scale.
In Situ Library Prep
Scalable workflow
Process 100s to 1M cells using a simple 1-day protocol
Cost
Achieves COGS below incumbents by reducing reagents and consumables
Extensible
Approach suitable for multi-omics: DNA, RNA, protein, methylation & more
Flexible
In situ library prep method supports hypothesis-free and hypothesis-driven applications
Platform Agnostic
In situ chemistry analogous to bulk NGS library prep & can be adapted to many instrument platforms
Ready to Start Your Single-Cell Study?
Whether you’re mapping somatic mosaicism across whole tissues or resolving complex cell engineering outcomes, Factorial provides the scale, sensitivity, and flexibility needed to transform discovery. Fill out the form below to contact our team and begin planning your next single cell multi-omic experiment.